Phosphotyrosine (HRP)

The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.\n\nThe binding of P4078-01E to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine.\n\nThe affinity of P4078-01E for phosphotyrosine is 10e-6 to 10e-7 M.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000-1:4,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\nLabeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

P4078-01E

Size

500ul

Applications

ELISA

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 50% glycerol. Labeled with horseradish peroxidase (HRP).

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG2b

References

1. Ruff-Jamison, S. et al. (1991) Heavy and light chain variable region sequences and antibody properties of anti-phosphotyrosine antibodies reveal both common and distinct features. J. Biol. Chem. 266: 6607-6613. 2. Takagi, S. et al. (1991) Intracellular localization of tyrosine kinase substrates beneath crosslinked surface immunoglobulins in B cells. J. Exp. Med. 174: 381-388. 3. Glenney, J.R. Jr. et al. (1988) Monoclonal antibodies to phosphotyrosine. J. Immunol. Methods. 109: 277-285.

Additional Info

Recognizes phosphotyrosine.