PI3 Kinase Class II Alpha (Class II Phosphatidylinositol 3-kinases, PI3KCII-alpha)

Class II phosphatidylinositol 3-kinases (PI3K) contain a C-terminal C2 domain that is unique to the class II isoforms of the PI3K family. This C2 domain mediates protein and phospholipid binding acitivities (1,2). PI3K Class II a generates phosphatidylinositol 3-phosphate (PIP3) and phosphatidylinositol 3,4-bisphosphate (PI(3, 4)P2) from phosphatidylinositol and phosphatidylinositol 4-phosphate (3). PI3K Class II a is located in various intracellular locations such as the trans-Golgi network, endocytic compartments, clathrin-coated vesicles, and nuclear speckles (1,4,5). Research studies have indicated that PI3K Class II a regulates the assembly and distribution of clathrin, resulting in the modulation of clathrin-dependent trafficking and sorting within the trans Golgi network (5,6). PI3K Class II a also mediates translocation of the glucose transporter GLUT4 to the plasma membrane in response to insulin (7). PI3K Class II a has also been shown to regulate neurosecretory granule exocytosis (8) and vascular smooth muscle contraction (9). Unlike other PI3K family members, PI3K Class II a is less sensitive to the PI3K inhibitors wortmannin and LY294002 (3).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137481

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Didichenko, S.A. and Thelen, M. (2001) J Biol Chem 276, 48135-42. 2. Stahelin, R.V. et al. (2006) J Biol Chem 281, 39396-406. 3. Domin, J. et al. (1997) Biochem J 326 (Pt 1), 139-47. 4. Domin, J. et al. (2000) J Biol Chem 275, 11943-50. 5. Gaidarov, I. et al. (2001) Mol Cell 7, 443-9. 6. Gaidarov, I. et al. (2005) J Biol Chem 280, 40766-72. 7. Falasca, M. et al. (2007) J Biol Chem 282, 28226-36. 8. Wen, P.J. et al. (2008) Mol Biol Cell 19, 5593-603. 9. Yoshioka, K. et al. (2007) Mol Pharmacol 71, 912-20.

Additional Info

Recognizes endogenous levels of total human PI3K Class II Alpha. Species Crossreactivity: mouse, rat, monkey.