PI4K2A, NT (Phosphatidylinositol 4-kinase Type 2 alpha, PIK42A, Phosphatidylinositol 4-kinase Type II-alpha, PI4KII, DKFZp761G1923, RP11-548K23.6) (APC)

Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs. PI4K II is activated by detergent and inhibited by adenosine. Overexpression of PI4KII in COS-7 cells increases synthesis of PtdIns4P. Some cells overexpressing PI4KII have scattered or no perinuclear Golgi. Knockdown of PI4KII by RNA interference (RNAi) does not disrupt the Golgi, and some cells show expanded Golgi. RNAi reduces the Golgi level of PtdIns4P and blocks the association between AP1 and the trans-Golgi network. PI4KII RNAi had little effect on intra-Golgi trafficking, but it inhibited export to plasma membrane export by 35%. It has been proposed that PI4KII generates PtdIns4P-rich domains within the Golgi that specify docking of the AP1 coat machinery.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:1000\nWestern Blot: 1:100-1:500\nImmunohistochemistry: 1:50-1:100\nImmunofluorescence: 1:10-1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4186-27P-APC

Size

200ul

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Wang, Y.J., et al., Cell 114(3):299-310 (2003). 2. Minogue, S., et al., J. Biol. Chem. 276(20):16635-16640 (2001). 3. Barylko, B., et al., J. Biol. Chem. 276(11):7705-7708 (2001). 4. Waugh, M.G., et al., Biochem. J. 373 (Pt 1), 57-63 (2003).

Additional Info

Recognizes human PI4K2A.

Alternative Names

Phosphatidylinositol 4-kinase Type 2-alpha, EC=2.7.1.67