PIM2tide (BAD aa107-117)

Synthetic peptide (RSRHSSYPAGT) (linear) corresponding to aa107- 117 of mouse BAD.\n\nHematopoietic growth factors mediate the survival and proliferation of blood-forming cells, but the mechanisms through which these proteins produce their effects are incompletely known. Recent studies have identified the pim family of kinases as mediators of cytokine-dependent survival signals. Several studies have identified substrates for the pim-1 kinase, but little is known about the other family members, pim-2 and pim-3. Pim-2 mRNA and protein expression are regulated by cytokines similarly to pim-1. Three PIM-2 protein isoforms are produced in cytokine-treated cells. All three forms are active kinases, and the short (PIM-2, 34kD) form is the most active at enhancing survival of FDCP1 cells after cytokine withdrawal. This pro-survival function involves inhibition of apoptosis and caspase activation. Enforced expression of PIM-2, 34kD) kinase does not appear to regulate expression of BCL-2, BCL-xL, BIM, or BAX proteins. However, the kinase can phosphorylate the pro-apoptotic protein BAD on serine 112, which accounts in part for its ability to reverse Bad-induced cell death. Our results indicate that pim-2 functions similarly to pim-1 as a pro-survival kinase and suggest that BAD is a legitimate PIM-2 substrate.\n\nApplications:\nKinase Assay: 300uM of this lot was phosphorylated using 50-200ng of PIM2, active\n\nStorage and Stability: \nLyophilized powder may be stored at 4 degrees C for short-term only. Stable for 12 months at -20 degrees C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.\n\n\n

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SPECIFICATIONS

Catalog Number

P4186-63

Size

1mg

Form

Supplied as a lyophilized powder.

Purity

> 95%

References

General References:\nJ Biol Chem. 2003 Nov 14;278(46):45358-67. Epub 2003 Sep 3. The PIM-2 kinase phosphorylates BAD on serine 112 and reverses BAD-induced cell death. Yan B, Zemskova M, Holder S, Chin V, Kraft A, Koskinen PJ, Lilly M.\n