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PIN4, Recombinant, Human, aa1-156, His-tag (Peptidyl-prolyl Cis-trans Isomerase NIMA-interacting 4, Peptidyl-prolyl Cis-trans Isomerase Pin4, PPIase Pin4, Rotamase Pin4, Parvulin-14, Par14, Parvulin-17, Par17, Peptidyl-prolyl Cis/trans Isomerase EPVH, hEP

Cat no: P4190-11C

PIN4, Recombinant, Human, aa1-156, His-tag (Peptidyl-prolyl Cis-trans Isomerase NIMA-interacting 4, Peptidyl-prolyl Cis-trans Isomerase Pin4, PPIase Pin4, Rotamase Pin4, Parvulin-14, Par14, Parvulin-17, Par17, Peptidyl-prolyl Cis/trans Isomerase EPVH, hEP

PIN4, also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, is peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and is essential for cell cycle regulation. There are two different isoforms which are PAR14 and PAR17. PIN4/PAR17 was imported into mitochondria in a time and membrane potential-dependent manner, where it reached the mitochondrial matrix. Moreover, Pin4 protein was shown to bind to double-stranded DNA under physiological salt conditions.\n\nSource: \nRecombinant corresponding to aa1-156 from human PIN4, fused to His-tag at N-terminus expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MPMAGLLKGL VRQLERFSVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK\n\nMolecular Weight: \n~18.8kD (176aa), confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4190-11C

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 1mM DTT, 0.1mM PMSF, 10% glycerol.

Purity

~85% (SDS-PAGE)

References

1. Uchida T., et al. (1999) FEBS Lett. 446:278-282. 2. Fujiyama-Nakamura S., et al. (2009) Mol. Cell. Proteomics. 8:1552-1565.

Read more on Supplier website

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

More info
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