PIST (PDZ Protein Interacting Specifically with TC10, CFTR-associated ligand, CAL, dJ94G16.2, Fused in Glioblastoma, FIG, Golgi-associated PDZ and Coiled-coil Motif Containing, Golgi Associated PDZ and Coiled Coil Motif Containing Protein, GOPC, GOPC1, OT

GOPC (FIG) was originally identified as a Golgi-associated, PDZ domain containing protein. It has two coiled-coil domains (CC1 and CC2) located in the amino-terminal region and a PDZ domain in the carboxy-terminal region (1). The CC2 domain and its adjacent linker region mediate the association of GOPC with the Golgi protein golgin-160 and the Q-SNARE protein syntaxin 6 (1,2). The PDZ domain of GOPC interacts with the carboxy terminus of target proteins to mediate target protein vesicular trafficking and surface expression (3-6). Fusion of the corresponding GOPC gene with the ROS tyrosine kinase oncogene has been detected in some glioblastoma. The resulting GOPC-ROS fusion protein is targeted to the Golgi apparatus where a constitutively activate ROS tyrosine kinase can mediate tumor formation (7,8). \n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. \nImmunoprecipitation: 1:50 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4207-51E

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 0.1%, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Charest, A. et al. (2001) J Biol Chem 276, 29456-65, 2. Hicks, S.W. and Machamer, C.E. (2005) J Biol Chem 280, 28944-51, 3. Cheng, J. et al. (2002) J Biol Chem 277, 3520-9, 4. He, J. et al. (2004) J Biol Chem 279, 50190-6, 5. Wente, W. et al. (2005) J Biol Chem 280, 32419-25, 6. Ito, H. et al. (2006) Biochem J 397, 389-98, 7. Charest, A. et al. (2003) Proc Natl Acad Sci U S A 100, 916-21, 8. Charest, A. et al. (2006) Cancer Res 66, 7473-81.

Additional Info

Recongnizes endogenous levels of human GOPC following IP enrichment. Species crossreactivity: rat. Sequence Homology: mouse