PKC theta, ID (Protein Kinase C theta Type, nPKC-theta, PRKCQ, PRKCT)

Protein kinase C (PKC) is a family of serine/threonine kinases that plays crucial roles in signal transduction. Members of the PKC family are divided into three groups based on their molecular structures and activating mechanisms: 1) Conventional PKC (a, b, and g) requiring calcium, phosphatidylserine (PS), and diacylglycerol (DG) for activation; 2) novel PKC (d, e, eta, and theta isoforms) activated independent of calcium; and 3) atypical PKC (Xi and lambda/Iota), which are independent of both calcium and DG. nPKC theta consists of 707aa residues and shows the highest sequence similarity to nPKC delta. nPKC theta has a zinc-finger-like cysteine-rich sequence (C1 region) and a protein kinase domain sequence (C3 region), both of which are common in all PKC family members. nPKC theta lacks a putative Ca+2 binding region (C2 region) that is seen only in conventional PKC subfamily. Analysis of mRNA from various human tissues revealed high-level expression of PKC-theta was found in skeletal muscle, lung, T cells, and brain, and minimal expression in cardiac muscle, placenta, and liver.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9103-68G

Size

50ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Choi, M. et al. J Biol Chem 278, 23610 (2003). 2. Niino, Y. et al. J Biol Chem 276, 36711 (2001). 3. Puceat. et al. J Biol Chem 269, 16938 (1994). 4. Chang, J. et al. J Biol Chem 268, 14208 (1993). 5. Osada, S. et al. Mol Cell Biol 3930, 9 (1992).

Additional Info

Recognizes human PKC theta.