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PKC (zeta)(P404) pAb

PKC (zeta)(P404) pAb

Cat no: BS1294

Supplier: Bioworld Technology, Inc.
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Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms ((alpha), (beta)I, (beta)ii and (gamma)) and novel (n) PKC isoforms ((delta), E , ?, n and 0). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC (delta) and E are independent of Ca++. On the other hand, nPKC (delta) and E, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities
Catalogue number: BS1294
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ug/100ul
Swiss prot: Q05513
Dilutions: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic peptide, corresponding to amino acids 380-420 of Human PKC ?.
Species: PKC (zeta)(P404) pAb detects endogenous levels of PKC (zeta)protein.
Molecular weight: ~ 70 to 85 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: Protein kinase C zeta type; nPKC-zeta; PRKCZ; PKC2; PKC zeta
Additional info: For research use only, not for use in diagnostic procedure.

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