PKD2, Phosphorylated (Ser197/Ser200) (Autosomal dominant polycystic kidney disease type II protein, Polycystic kidney disease 2 protein, Polycystwin, R48321)

As members of the calcium-/calmodulin-depedent protein kinase superfamily, there are three known Protein Kinase D (PKD) isoforms, PKD1, PKD2, and PKD3. These serine/threonine kinases are involved in protein-protein and protein-carbohydrate adhesion and are also thought to be ion channel regulators. They are prominent downstream targets of protein kinase C and phospholipase D in various biological systems including mouse. PKDs are activated by various stimuli, including phorbol esters, G-protein-coupled receptors, reactive oxygen species, and diacylglycerol. PKDs regulate protein transport in the trans-Golgi network and are thought to play a role in muscle differentiation, cell motility, migration, and invasion. These isoforms are known to interact with each other. PKD2 is shown to be activated by phorbol esters, both in vivo and in vitro, and also by gastrin via the cholescystokinin/CCK(B) receptor in stably-transfected AGS-B cells. Furthermore, PKD2 is found to be active during the early phase of differentation of C2C12 cells into skeletal muscle cells. It is also reported to enhance TCR-induced NFAT activity in Jurkat T cells.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMEF cell lysates untreated and PBDu-treated\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

029580

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Sturany, S., et al. (2002). J Biol Chem. 277(33):29431-29436. 2. PLoS One. 2011 Jan 27;6(1):e14599. PMID:21298052. 3. Li. Q., et al. (2009). BMB Rep. 42(1):35-40.

Additional Info

Recognizes mouse PKD2 phosphorylated at Ser197/Ser200. Species sequence homology: human, rat.