Plasminogen, Glu, Bovine

Plasminogen is a single chain glycoprotein zymogen which is synthesized in the liver and circulates in plasma at a concentration of ~2.4uM (1,2). The plasminogen molecule contains 790 amino acids, 24 disulfide bridges, no free sulfhydryls and 5 regions of internal sequence homology, known as kringles, between Lys77 and Arg560. These five triple-looped, three disulfide bridged, kringle regions are homologous to the kringle domains in t-PA, u-PA and prothrombin. Plasminogen contains one high affinity (Kd=9x10-6M) and four low affinity (Kd=5x10-3M) lysine binding sites. The high affinity binding site resides within the first kringle region of plasminogen. The interaction of plasminogen with fibrin and alpha2-antiplasmin is mediated by these lysine binding sites. Native glu-plasminogen (Mr=88,000) is readily converted to Lys-77-plasminogen (Mr=83,000) by plasmin hydrolysis of the Lys76-Lys77 peptide bond. Elastase catalyzed cleavage of the Val441-Val442 peptide bond of glu-plasminogen yields a functionally active zymogen termed Val-442 plasminogen or mini-plasminogen. \n\nThe conversion of plasminogen to plasmin occurs by a variety of mechanisms, but all result in hydrolysis of the Arg560-Val561 peptide bond of plasminogen, yielding two chains which remain covalently associated by a disulfide bond. \n\nActivity Determination\n(same/less than)10 ppm plasmin activity for glu-PG\n\nLocalization: Plasma\n\nPlasma Concentration: 2.4uM (human) (4)\n\nMode of action: Zymogen; precursor to the serine protease plasmin\n\nExtinction Coefficient: E 1%1cm, 280nm=17.0 (5)\n\nIsoelectric point: 6.2 (1)\n\nStructure: Single chain, 24 intra chain disulfide bridges, 5 kringle regions\n\nPercent carbohydrate: ~2%\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4256-51

Size

1mg

Form

Supplied as a liquid, 50% glycerol.

Purity

~95% (SDS-PAGE)

References

1. Robbins, K.C., Methods in Enzymology, 45, 257 (1976), 2. Collen, D. in Blood Coagulation, Zwaal, R.E.A. and Hemker, H.C., eds., pp. 243-258, New York, Elsevier (1986), 3. Castellino, F.J., et al., Methods in Enzymology, 80, 365 (1981), 4. Wohl, R.C., et al., Thromb. Res., 27 523 (1982), 5. Barlow, G.H., et al., Biochemistry, 23, 2384 (1984), 6. Sottrup-Jensen, L., et al., in Progress in Chemical Fibrinolysis and Thrombolysis, Vol. 3, ed. J.F. Davidson, 7. R.M. Rowan, M.M. Samana, P.C. Desnoyers, pp. 197-228, New York: Raven Press (1975), 8. Zaas AK, Liao G, Chien JW, Weinberg C, Shore D, et al. (2008) Plasminogen Alleles Influence Susceptibility to Invasive Aspergillosis. PLoS Genet 4(6): e1000101.doi:10.1371/journal.pgen.1000101 (mouse plasminogen).