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Platelet Derived Growth Factor Receptor, Type B (PDGFRb)

Cat no: P4258-20C

Platelet Derived Growth Factor Receptor, Type B (PDGFRb)

PDGFR (Platelet Derived Growth Factor Receptor) exhibits two different isoforms, alpha and beta coded by two different genes. They possess five immunoglobulin like domains that are involved in ligand binding. Two tyrosine kinase domains are separated by a kinase insert to which PI-3 kinase can bind. PDGF AA can bind autophosphorylate and activate only PDGFR, alpha while PDGF BB can bind and activate to both PDGFR, alpha and beta.\n\nApplications:\nSuitable for use in Immunohistochemistry (Formalin/paraffin). Other applications not tested. \n\nRecommended Dilution: \nImmunohistochemistry: 5-10ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\nOptimal dilution determined by the researcher. \n\nPositive Control: Ovarian carcinoma.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4258-20C

Size

250ul

Applications

IHC

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Matsui T, et al. (1989) Science, 243:800-804.\n2. Claesson-Welsh L, et al. (1989) Proc Natl Acad Sci, 86:4917-4921.

Additional Info

Recognizes human PDGFR, beta. Species Crossreactivity: mouse.

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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