Platelet Derived Growth Factor Receptor Type B (PDGFRb)

Two types of PDGF receptors have been identified: PDGF receptor A (PDGFRa) and PDGF receptor B (PDGFRb). The PDGFRa, a 170kD protein, binds all three isoforms of PDGF with high affinity whereas the PDGFRb, a 190kD protein, appears to bind only the PDGF BB homodimer with high affinity. Both receptors are transmembranous, ligand-activated protein tyrosine kinases, which phosphorylate a number of important signal transduction proteins, which are bound with differential affinities via SH2 domains. The response of any given cell to PDGF will depend on the types of receptors displayed on the surface and isoforms of PDGF present in the extracellular environment.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml detects PDGFb in a mouse 3T3/A31 cell RIPA lysate. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P4258-23 (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. \nImmunoprecipitation: 5ug immunoprecipitates PDGFb in a mouse 3T3/A31 cell RIPA lysate.\nOptimal dilutions to be determined by the researcher.\n\nPositive Antigen Control Included: \nF4209: 3T3/A31 cell lysate. Use 20ug per lane for minigels.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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