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PLK1 (D204) pAb

PLK1 (D204) pAb

Cat no: BS1687


Supplier: Bioworld Technology, Inc.
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PLK1 (polo-like kinase 1) is a member of the serine/threonine protein kinase family, cdc5/polo subfamily. PLK1 contains two polo box domains with a predicted molecular weight of 68 kDa. PLK1 has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. PLK1 is modified by phosphorylation at Threonine 210. PLK1 may also be required for cell division. Depletion of PLK1 results in apoptosis and deregulation of expression of PKL1 is correlated with development of many malignancies. Studies have shown that ab14209 preferentially detects the kinetochore-associated pool of Plk1. An alternative antibody, Mouse monoclonal to Plk1 (ab14210) preferentially stains centrosomal-associated Plk1.
Catalogue number: BS1687
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ug/100ul
Swiss prot: P53350
Dilutions: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic peptide, corresponding to amino acids 180-220 of Human PLK1.
Species: PLK1 (D204) pAb detects endogenous levels of PLK1 protein.
Molecular weight: ~ 70 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: PLK1; EC 2.7.11.21; PLK; PLK-1; STPK13
Additional info: For research use only, not for use in diagnostic procedure.

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