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Pluripotent Stem Cell 3-Color\nImmunocytochemistry Kit, Human, BioAssay(TM)

Cat no: P4285

Pluripotent Stem Cell 3-Color\nImmunocytochemistry Kit, Human, BioAssay(TM)

This kit contains three fluorochrome conjugated antibodies that can be used for single-step immunocytochemical staining of human pluripotent stem cells (including embryonic and induced pluripotent stem cells). Each antibody is supplied as 12.5ug in 125ul of PBS containing 0.1% sodium azide.\n\nKit Components:\nAnti-human SOX2 NL557-Conjugated Goat IgG \nAnti-human Oct-3/4 NL637-Conjugated Goat IgG\nAnti-human Nanog NL493-Conjugated Goat IgG\n\nIntended Use:\nThis product is designed for the immunocytochemical analysis of human pluripotent stem cells using three fluorochrome-conjugated antibodies.\n\nImmunocytochemistry Validation:\nThese antibodies have been tested for immunocytochemistry staining using BG01V cells grown on irradiated mouse embryonic fibroblasts. Cells were fixed in PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.3% Triton(R) X-100, and 1% BSA. After blocking, cells were incubated for 3 hours at RT in the dark with all 3 conjugated antibodies at a final concentration of 10ug/ml each (1:10 dilution) in blocking buffer. Between each step, cells were washed with PBS containing BSA. When using a staining volume of 50ul, this kit contains 25 tests.\n\nFluorochrome Absorption Maximum (nm) Emission Maximum (nm)\nNL557 557 574\nNL637 637 658\nNL493 493 514\n\nTypical Data:\nBG01V cells grown on irradiated mouse embryonic fibroblasts were simultaneously stained with (A) NL637- conjugated Oct-3/4, (B) NL493-conjugated Nanog, and (C) NL557-conjugated SOX2. All nuclei were stained with DAPI (shown in panel D).\n\nStorage and Stability:\nStore at 2-8 degrees C in the dark. Use within 6 months of receipt.\n\nBG01V cells are licensed from Novocell, Inc. Triton is a registered trademark of Union Carbide.

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SPECIFICATIONS

Catalog Number

P4285

Size

25Tests

Applications

ICC

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