PML (P39) pAb

The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t(15;17) (q22;q11.2-q12) chromosomal trans-location of the retinoic acid receptor (alpha) (RAR(alpha)) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RAR(alpha) chimeric protein. Myeloid precursor cells expressing the PML/ RAR(alpha) chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RAR(alpha) transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/ RAR(alpha) also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis.

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SPECIFICATIONS

Catalog Number

BS1296

Size

100ug/100ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to the N-terminal of Human PML.

Species

PML (P39) pAb detects endogenous levels of PML protein.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200\nIF: 1:50~1:200

Swiss Prot

P29590

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul