Polynucleotide Kinase T4

T4 polynucleotide kinase catalyzes the transfer of the terminal phosphate of ATP (gamma-orthophosphate) to the 5'-hydroxyl termini of ribo- and deoxyribonucleotides. In the presence of ADP the enzyme also catalyzes an exchange reaction. In the exchange reaction, an excess ADP causes the enzyme to transfer the 5' terminal phosphate from phosphorylated DNA to ADP. The DNA thus dephosphorylated is then rephosphorylated by transfer of radiolabeled gamma-phosphate from gamma 32P ATP.\n\nApplications:\nApplications:\n

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SPECIFICATIONS

Catalog Number

P4410

Size

500U

References

1. Richardson, C.C., The Enzymes (Boyer, P.D., ed.), 14: 299-314, Academic Press, San Diego, 1981. 2. Sambrook, J., Russel, D.W., Molecular Cloning: A Laboratory Manual, 3rd. Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2001. 3. Current Protocols in Molecular Biology, Vol. 1 (Ausubel, F.M., et al., ed.), John Wiley & Sons, Inc., Brooklyn, New York, 3.10.2-3.10.5, 1994-2001. 4. Berkner, K.L., Folk, W.R., J. Biol. Chem. 252: 3176-3184, 1977. 5. Harrison, B., Zimmerman, S.B., Anal. Biochem. 158: 307-315, 1986. 6. Phillips, D.H., Mutation Res. 378: 1-12, 1997. 7. Keith, G., Dirheimer, G., Curr. Opin. Biotechnol. 6: 3-11, 1995.

Alternative Names

EC=2.7.1.78; E. coli T4 phage; Supplied with Forward, Exchange Reaction Buffers, PEG Solution