Postsynaptic Density Protein 95 (PSD-95, PSD95, Discs Large Homolog 4, DLG4, (Drosophila) Post-synaptic Density Protein 95, Synapse-associated Protein 90, SAP90, SAP-90)

Postsynaptic Density Protein 95 (PSD-95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex. These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins. Thus different PDZ domains of PSD-95 might be specialized for distinct functions. PSD-95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning. Mutations in PSD-95 are associated with autism.\n\nApplications:\nSuitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:250. Detects an ~100kD band corresponding to the apparent molecular mass of PSD-95 on SDS-PAGE blots. An additional band of >100kD is also detected. Additional crossreactive bands are detected at ~75kD and 50kD in rat and mouse samples.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4700-08D

Size

25ug

Applications

ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a,k

References

1. Chetkovich, D.M., et al., J. Neurosci. 22(15): 6415-6425 (2002). 2. Cao, J., et al., J. Cell Biol. 168(1): 117-126 (2005). 3. Kennedy, M., Trends in Neurosci. 6: 264-268 (1997). 4. Irie, M., et al., Science 277(5331): 1511-1515 (1997). 5. Cai, C., et al., J. Biol. Chem. 281: 4267-4273 (2006). 6. Yao, W.D., et al., Neuron 41: 625-638 (2004). 7. Cline, H., Curr Biol. 15: R203-205 (2005).

Additional Info

Recognizes rat Postsynaptic Density Protein 95 at ~100kD. \nSpecies Crossreactivity: bovine, human and mouse