Postsynaptic Density Protein 95 (PSD-95, PSD95, Discs Large Homolog 4, DLG4, Post-synaptic Density Protein 95, Synapse-associated Protein 90, SAP90, SAP-90) discontinued

Postsynaptic Density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an Nterminal variable segment followed by three aminoterminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).\n\nApplications:\nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 4ug/ml was sufficient for detection of PSD-95 in 20ug of rat brain tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.\nOptimal dilutions to be determined by the researcher. \n\nRecommended secondary antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\nI1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4700-02

Size

25ug

Applications

ICC, WB

Hosts

Mouse

Reactivities

Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% azide, and 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Chetkovich D.M., et al (2002) J Neurosci. 22(15): 6415-25. 2. Cao J., et al(2005) J Cell Biol. 168(1): 117-26. 3. Kennedy M. (1997) Trends in Neurosci. 6: 264-268. 4. Irie M. et al. (1997) Science 277(5331): 1511-5. 5. Cai C. et al. (2006) J Biol Chem. 281: 4267-73. 6. Yao W.D. et al. (2004) Neuron 41: 625-38. 7. Cline H. (2005) Curr Biol. 15: R203-5.

Additional Info

Recognizes an ~100kD protein corresponding to the apparent molecular mass of rat PSD-95 on SDS-PAGE immunoblots. An additional protein of > 100kD is also detected. Additional crossreactive bands are detected at ~75kD and 50kD in rat and mouse samples. Species Crossreactivity: mouse, bovine.