Postsynaptic Density Protein 95 (PSD-95, PSD95, Discs Large Homolog 4, DLG4, Post-synaptic Density Protein 95, Synapse-associated Protein 90, SAP90, SAP-90)

Postsynaptic density protein 95kD (PSD-95), also known as Synapse associated protein 90kD (SAP90), is a brain specific protein that is highly similar to the Drosophila dlg tumor suppressor protein (1). PSD-95 is a member of membrane associated proteins that are localized beneath the postsynaptic membrane of synapses in the CNS. PSD-95 contains a carboxyl-terminal guanylate kinase domain, an upstream SH3 domain, and three amino-terminal PDZ domains. PSD-95 interacts with NMDA receptor and Shaker-type K+ channel and contributes to their clustering and localization at the synaptic spines in hippocampal neurons and the pinceau terminal of cerebellar basket cells, respectively (2). The yeast two hybrid method revealed that the second PDZ domain in PSD-95 binded tightly to the carboxyl terminal (t)-S/TXV sequence of the NR2B subunit of NMDA receptor and of Shaker-type K+ channel. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interaction between PDZ domains present on both proteins.\n\nApplications:\nWestern Blot (ECL): 5 ug/ml\nImmunoctyochemistry (3,4): 12.5 ug/ml 1:500\nOptimal dilutions to be determined by researcher.\n\nPositive Controls:\nMouse Brain Tissue Extract\nRat Brain Tissue Extract

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SPECIFICATIONS

Catalog Number

P4700-05

Size

25ug

Applications

WB

Hosts

Mouse

Reactivities

Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, pH7.2, 0.1mM PMSF, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Cho, K.O., Hunt, C.A. , Kennedy, M.B. (1992) Neuron 9: 929-942.\n2. Kennedy, M. (1997) Trends in Neurosci. 6: 264-268.\n3. Kornau, H.C., et al. (1995) Science 269: 1737-1740.\n4. Li, A.J., Suzuki, S., Suzuki, M., Mizukoshi, E., Imamura, T. (2002) Eur J Neurosci 16(7):\n1313-1324.\n

Additional Info

Detects a ~100kD protein, corresponding to the apparent molecular mass of PSD-95 on SDS-PAGE immunoblots, in samples from mouse, rat and bovine. A molecular mass band of ~75kD is also present in rat brain and mouse brain tissue extracts.\n