Postsynaptic Density Protein 95 (PSD-95, PSD95, Discs Large Homolog 4, DLG4, Post-synaptic Density Protein 95, Synapse-associated Protein 90, SAP90, SAP-90)

Postsynaptic density protein 95kD (PSD-95), also known as Synapse associated protein 90kD (SAP90), is a brain specific protein that is highly similar to the Drosophila dlg tumor suppressor protein. PSD-95 is a member of membrane associated proteins that are localized beneath the postsynaptic membrane of synapses in the CNS. PSD-95 contains a carboxyl-terminal guanylate kinase domain, an upstream SH3 domain, and three amino-terminal PDZ domains. PSD-95 interacts with NMDA receptor and Shaker-type K+ channel and contributes to their clustering and localization at the synaptic spines in hippocampal neurons and the pinceau terminal of cerebellar basket cells, respectively. The yeast two hybrid method revealed that the second PDZ domain in PSD-95 binded tightly to the carboxyl terminal (t)-S/TXV sequence of the NR2B subunit of NMDA receptor and of Shaker-type K+ channel. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interaction between PDZ domains present on both proteins. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilutions: \nWestern Blot (ECL): 1:1000 \nOptimal dilution determined by the researcher. \n\nPositive Controls:\nRat Brain Tissue Extract, Mouse Brain Tissue Extract. Bovine Brain Tissue Extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4700-10

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Cho, K.O., Hunt, C.A. and Kennedy, M.B. (1992) Neuron 9: 929-942. 2. Kennedy, M. (1997) Trends in Neurosci. 6: 264-268. 3. Kornam, H.C., et al. (1995) Science 269: 1737-1740. 4. Kornau, H.C., Schenker, L.T., Kennedy, M.B., Seeburg, P.H. (1995) Science 269(5231): 1737-1740.

Additional Info

Recognizes rat PSD95 at ~95kD. Species Crossreactivity: mouse and bovine.