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PRNP, Recombinant, Human, aa23-230, His-Tag (Prion Protein Preproprotein, ASCR, CD230, CJD, GSS, MGC26679, Prion, PRIP, PrP, PrP27-30, PrP33-35C, PrPc)

Cat no: 137170

PRNP, Recombinant, Human, aa23-230, His-Tag (Prion Protein Preproprotein, ASCR, CD230, CJD, GSS, MGC26679, Prion, PRIP, PrP, PrP27-30, PrP33-35C, PrPc)

Prion protein, also known as PRNP, is a ubiquitous membrane glycoprotein whose abnormal self- replicating, misfolded form is widely believed to cause several central nervous system disorders, collectively known as Transmissible Spongiform Encephalopathies (TSE). This protein contains a highly unstable region of five tandem octapeptide repeat. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. \n\nSource: \nRecombinant protein corresponding to human PRNP, fused to His-tag at N-terminal expressed in E.coli.\n\nMolecular Weight: \n~25kD (229aa)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MKKRPKPGGW NTGGSRYPGQ GSPGGNRYPP QGGGGWGQPH GGGWGQPHGG GWGQPHGGGW GQPHGGGWGQ GGGTHSQWNK PSKPKTNMKH MAGAAAAGAV VGGLGGYVLG SAMSRPIIHF GSDYEDRYYR ENMHRYPNQV YYRPMDEYSN QNNFVHDCVN ITIKQHTVTT TTKGENFTET DVKMMERVVE QMCITQYERE SQAYYQRGS\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

137170

Size

20ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 1M urea, 10% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Weiss S., et al. (1996) Biochem Biophys Res Commun. 219:173-179. 2. Lee I Y., et al. (1998) Genome Res. 8:1022-1037.

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

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Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

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Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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