Programmed cell death protein 1 (Protein PD-1/mPD-1, PDCD1)

PDCD1(Programmed cell death 1), also called PD1, encodes a cell surface receptor that is a member of the B7 superfamily involved in immunomodulation. PDCD1 acts as an inhibitory molecule on T cells after interacting with its ligands PDL1 and PDL2. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. The PDCD1 gene is mapped on 2q37.3. The PDCD1 gene contains 5 exons. In mouse cells, the Pdl2 protein bound to human PDCD1, resulting in inhibition of T-cell proliferation and cytokine production. Using flow cytometric analysis, Said et al. found that expression of PDCD1 was upregulated on CD16-positive and CD16-negative monocytes, but not on dendritic cells, in viremic HIV-positive patients, but not in highly active antiretroviral therapy (HAART)-treated HIV-positive patients. PDCD1 upregulation in monocytes was induced by microbial Toll-like receptor ligands and inflammatory cytokines.\n\n\n\nUniProt Number:\nQ02242\n\nGene ID:\nPDCD1\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145091

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Barber, D. L., Wherry, E. J., Masopust, D., Zhu, B., Allison, J. P., Sharpe, A. H., Freeman, G. J., Ahmed, R. Restoring function in exhausted CD8 T cells during chronic viral infection. Nature 439: 682-687, 2006.\n2.Dai, H., Wan, N., Zhang, S., Moore, Y., Wan, F., Dai, Z. Cutting edge: programmed death-1 defines CD8+CD122+ T cells as regulatory versus memory T cells. J. Immun. 185: 803-807, 2010.\n3.Finger, L. R., Pu, J., Wasserman, R., Vibhakar, R., Louie, E., Hardy, R. R., Burrows, P. D., Billips, L. G. The human PD-1 gene: complete cDNA, genomic organization, and developmentally regulated expression in B cell progenitors. Gene 197: 177-187, 1997.\n

Additional Info

Recognizes mouse and rat PDCD1. No crossreactivity with other proteins.