Prolactin, Recombinant, Human (LTH, Luteotropic Hormone, Lactogenic Hormone)

Prolactin is a lactogenic hormone secreted by the adenohypophysis. Besides its major action on lactation, in some species prolactin exerts effects on reproduction, maternal behavior, fat metabolism, immunomodulation and osmoregulation. Prolactin has been shown also to have cytokine-like activities and to have important immunoregulatory activities. It contributes to the development of lymphoid tissues and the maintenance of physiological immune function and also modulates a variety of T-cell immune responses. Prolactin has been reported to activate cellular proliferation in nonreproductive tissue, such as liver, spleen, and thymus. It induces significant proliferation in aortic smooth muscle cells and also enhances proliferation of these cells induced by PDGF. Prolactin also appears to be directly mitogenic for pancreatic beta cells. Prolactin is also mitogenic for cultured astrocytes.\n\nRecombinant Human Prolactin is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of ~23kD.\n\nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ile-Cys.\n\nBiological Activity:\nProlactin is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of rat lymphoma, Nb2-11 was found to be < 0.065ng/ml.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9009-27

Size

10ug

Form

Supplied as a lyophilized powder in 10mM sodium phosphate, pH 8.0, 50mM sodium chloride. Reconstitute in sterile ddH2O, 0.1% BSA or HSA to (same/more than)100ug/ml

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Mechanism for ordered receptor binding by human prolactin. Sivaprasad U, Canfield JM, Biochemistry 2004 Nov 2;43(43):13755-65 2. A mutant receptor with enhanced dominant-negative activity for the blockade of human prolactin signalling. Flynn A, Whittington H, Uney J, J Mol Endocrinol 2004 Apr;32(2):385-96 3. Endogenous human prolactin and not exogenous human prolactin induces estrogen receptor alpha and prolactin receptor expression and increases estrogen responsiveness in breast cancer cells. Gutzman JH, Miller KK, J Steroid Biochem Mol Biol 2004 Jan;88(1):69-77 4. Differential effects of light/dark recombinant human prolactin administration on the submaxillary lymph nodes and spleen activity of adult male mice. Esquifino AI, Arce A, Chacon F, euroimmunomodulation 2004;11(2):119-26 5. Human prolactin and its antagonist, hPRL-G129R, regulate bax and bcl-2 gene expression in human breast cancer cells and transgenic mice. Peirce SK, Chen WY, Oncogene 2004 Feb 12;23(6):1248-55 6. The N-terminus of human prolactin modulates its biological properties. Bernichtein S, Jomain JB, Goffin V, Mol Cell Endocrinol 2003 Oct 31;208(1-2):11-21.