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Prostatic Acid Phosphatase (Prostatic Acid Phosphotase, PAP, Acid Phosphatase Prostate, ACPP, ACP3, ACP-3)

Cat no: P9054-73A

Prostatic Acid Phosphatase (Prostatic Acid Phosphotase, PAP, Acid Phosphatase Prostate, ACPP, ACP3, ACP-3)

ACPP is an ~50kD glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95-100kD nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP. This generates adenosine which acts as a strong analgesic agent. Mature human ACPP is 354aa in length (aa33-386). It contains one histidine phosphatase domain (aa34-332), plus a nucleophile acceptor site at His44, and a proton donor site at Asp290. There are two potential alternative splice variants. One shows a deletion of aa153-185, while another is transmembrane (previously called TMPase) and shows a 38aa substitution for the C-terminal seven aa. Over aa33-379, human ACPP shares 84aa identity with mouse ACPP.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2ug/ml\nImmunohistochemistry: 8-25ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9054-73A

Size

100ug

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human Prostatic Acid Phosphatase. No cross-reactivity with recombinant human TRACP/PAP/ACP5.

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