Protein C BioAssay(TM) ELISA Kit (Antibodies only)

Protein C (PC) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of PC in plasma is ~4ug/ml (~60 nM). A deficiency of Protein C (quantitative or qualitative) is a risk factor for vascular thrombosis. Protein C is expressed as a two-chain molecule with a molecular weight of 62 kD. The light chain (21kD of PC consists of two EGF-like domains and an amino-terminal domain containing one hydroxyaspartic acid and 11 g-carboxyglutamic acid (gla) residues. These residues allow PC to bind to membranes that contain acidic phospholipids in a calcium dependent manner. The heavy chain of PC (41 kD) consists of the catalytic domain and an activation peptide. Activation of Protein C results from cleavage at residue Arg12 in the heavy chain by a complex of thrombin and a cell surface cofactor thrombomodulin. \n\nThe activation of PC is associated with the release of a small activation peptide (2-3 kD, called Protein C peptide, or PCP) from the N-terminal of the heavy chain. Activated Protein C (APC) is a serine protease with anticoagulant activity. APC, in complex with a phospholipid membrane, calcium and the Protein S cofactor, exhibits anticoagulant activity through the proteolytic inactivation of coagulation cofactors Va and VIIIa. The primary inhibitor of APC activity in plasma is Protein C Inhibitor (PCI, also called Plasminogen Activator Inhibitor-3, PAI-3) and to a lesser extent by a1antitrypsin and a2macroglobulin. The inhibitory activity of PCI is stimulated approximately 10 fold by heparin1-3.\n\nMaterials Supplied:\nAntibodies for 5x96 well plates\n\nKit Components:\nP9102-38A: PC Capture Antibody: 1x500ul Supplied as a liquid in 50% glycerol. Purified by immunoaffinity chromatography. PS IgG Pab ShxHu for coating ELISA plates.\n\nP9108-07B: PC (HRP) Dection Antibody: 1x500ul Supplied as a liquid in 50% glycerol. Purified by immunoaffinity chromatography. PS (HRP) GtxHu for detection of captured PS.\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9102-38

Size

1Kit

Applications

ELISA

References

1) Bertina RM; Assays for Protein C, in Bertina RM (ed): Protein C and Related Proteins, Contemporary Issues in\nHaemostasis and Thrombosis #3, Churchill Livingston, New York, 1988, p 130.\n2) Soria J, Soria C, Samama M, Nicolas G, Kisiel W; Severe Protein C Deficiency in Congenital Thrombotic Disease -\nDescription of an Immunoenzymological Assay for Protein C Determination. Thrombosis and Haemostasis 53:3, p\n293-296, 1985.\n4) Harlow E, Lane D; Detecting and Quantitating Antigens using the Two-Antibody Sandwich Assay, in\nAntibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, 1988, p 580.