Protein C Receptor, Endothelial (Endothelial Cell Protein C Receptor, EPCR, Activated Protein C Receptor, APC Receptor, APCR, CCCA, CCD41, CD201, CD201 Antigen, Centrocyclin, MGC23024, PROCR)

Blood coagulation depends on a series of sequential proteolytic processes, leading to the generation of new serine proteases. Several natural anticoagulant mechanisms are balancing the clotting system. One of them is the protein C anticoagulant pathway. In this pathway, thrombin binds to the endothelial cell receptor thrombomodulin. This complex activates protein C to generate the anticoagulant enzyme activated protein C (APC) that when complexed to protein S inhibits coagulation by inactivating two critical regulatory proteins: factor Va and VIIIa.3. Protein C activation by the thrombin/ thrombomodulin complex is a relatively low-affinity reaction. However, it was found that protein C and APC bind to culture endothelial cells with a relatively high affinity. The high affinity binding of both ligands (Kd = 30nM) is mediated by EPCR (endothelial cell protein C receptor). EPCR is a type 1 transmembrane glycoprotein containing two domains in the extra cellular region that are homologous to the & amp;#65533;1 and & amp;#65533; 2 domains of CD1/MHC class 1 molecules.4 The mature human EPCR protein contains 211 amino acids.\nThe protein migrates as a 49kD protein due to its carbohydrates chains.1 The protein is expressed in endothelial cells present in various types of vessels including arterial heart, lung, and in the vena cava inferior. The EPCR is also expressed in small vessels such as capillaries of the alveolar wall in the lung. The high-affinity binding of protein C to EPCR is a critical step for activation. \n\nApplications: \nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 5-20ug/ml\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9102-39F

Size

100ul

Applications

FC, IHC

Hosts

Rat

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Ye, X., et al., Biochem. Biophys. Res. Commun., 259, 671-677 (1999). 2. Shu, F., et al., FEBS Lett., 477, 208-212 (2000). 3. Stearns-Kurosawa, D.J., et al., Proc. Natl. Acad. Sci. USA, 93, 10212-10216 (1996). 4. Fukudome, K., et al., J. Exp. Med., 187, 1029-1035 (1998). 5. Oganesyn, V., et al., J. Biol. Chem., 277, 24851-24854 (2002).

Additional Info

Recognizes human EPCR.