Protein Kinase A, NT (PKA)

The action of many hormones is mediated by the generation of the intracellular second messenger cAMP. The predominant effect of cAMP is to activate PKA (cAMP-dependent protein kinase). Four molecules of cAMP bind each dormant PKA holoenzyme activating the kinase by releasing the catalytic (C) subunits from the regulatory (R) subunit-cAMP complex. The cellular location of PKA is dictated by the R subunit. Two classes of R subunit exist; RI and RII, which form the type I and type II PKA holoenzymes respectively. The RI isoforms (RIa and RIb) are thought to be primarily cytoplasmic whereas a significant proportion of the RII isoforms (RIIa and RIIb) are particular and associate with the plasma membrane, cytoskeletal components, endoplasmic reticulum, secretory granules or nuclei.\n\nApplications: \nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml detected PKA, NT (42kD) in both rat brain microsomal preparation and PC-12 RIPA cell lysates. Rat brain preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with P9102-91A (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies: \nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb \nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9102-91A

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Prc

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

Additional Info

Recognizes PKA purified from bovine heart and recombinant human alpha isoform of PKA expressed in CHO cells. \nSpecies Crossreactivity: mouse, rat, hamster, bovine and porcine. Crossreactivity with the beta-isoform of mammalian PKA is possible since it shares identity in 14 of 15 residues.