Protein Kinase C, beta 2, phosphorylated (Ser660)

PKC beta II is a member of the serine/threonine protein kinase C (PKC) family (1) that includes 10 closely related isozymes classified as the conventional, the novel and the atypical. The conventional PKC beta II is implicated in diverse cellular process including secretion, proliferation, differentiation, apoptosis, permeability, migration & hypertrophy (2-3). PKC beta II structure consists of a C-terminal catalytic region, containing an activator-binding domain and a Ca2+-binding domain, and a regulatory domain. It has been shown that PKC beta II must first be processed by three distinct phosphorylations before it is competent to respond to second messengers. Specifically, the threonine at position 500 (T500) on the activation loop, and T641 and S660 on the carboxyl terminus of protein kinase C beta II are phosphorylated in vivo. T500 and S660 are selectively dephosphorylated in vitro by protein phosphatase 2A to yield an enzyme that is still capable of lipid-dependent activation, whereas all three residues are dephosphorylated by protein phosphatase 1 to yield an inactive enzyme. (4).\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000 \nImmunohistochemistry: 1:100 \nmmunocytochemistry: 1:50 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

P9103-18C4

Size

100ul

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Coussens L, et al (986) Science;233(4766):859-66. 2. Mellor, H., et al (1998) Biochem. J. 332, 281-292 3. Nishizuka, Y. (1995) FASEB J. 9, 484-496 4. Keranen LM, et al. Curr Biol 5(12):1394-1403, 1995 \n

Additional Info

Recognizes human PKC beta II phosphorylated at serine 660. Species crossreactivity: mouse and rat.