Protein Kinase D3 (PKD3, PRKD3, EPK2, Protein Kinase C nu Type, PKCnu, PKC-nu, nPKC nu, nPKC-nu, PRKCN, Protein Kinase EPK2, Serine/threonine Protein Kinase D3)

PKCv, also known as PKD3, is a member of the protein kinase C (PKC) family of erine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation. PKCv is composed of 890aa residues and has 77.3% similarity to\nhuman PKCu (PKCu) and 77. 4% similarity to mouse PKD (the mouse homolog of PKCu) (1). The PKCv mRNA is ubiquitously expressed in various tissues. PKCv has two putative diacylglycerol binding C1 domains, suggesting that it may participate in a novel diacylglycerol-mediated signaling pathway (2). PKCv is translocated to the plasma membrane and activated by the diacylglycerol mimic phorbol 12-myristate 13-acetate. PKCv is an important\nphysiologic target of the B-cell receptor (BCR) and exhibits robust activation after BCR engagement (2). GPCR agonists induce a rapid activation of PKCv by a protein kinase C\n(PKC)-dependent pathway that leads to the phosphorylation of the activation loop of PKCv. PKCv is present both in the nucleus and cytoplasm and this distribution of PKCv results from its continuous shuttling between both compartments by a mechanism that requires a nuclear import receptor and a competent CRM1-nuclear export pathway (3). Cell stimulation with the GPCR agonist neurotensin induces a rapid and reversible plasma membrane translocation of PKCv that is PKC-dependent.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS,\n0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9103-73L3

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Hayashi, A. et al. (1999) Biochim. Biophys. Acta. 1450, 99-106. (2) Matthews, S.A. et al. (2003) J. Biol. Chem. 278, 9086-91. (3) Rey, O. et al. (2003) J. Biol. Chem. 278, 23773-85.

Additional Info

Recognizes endogenous levels of total human PKD3/PKCv. Species Crossreactivity: bovine, mouse, monkey, rat.

Alternative Names

EC=2.7.11.13