PTGDS2, aa1-199, Recombinant, Human (Hematopoietic Prostaglandin D Synthase, H-PGDS, GST Class-sigma, Glutathione S-transferase, Glutathione-dependent PGD Synthase, Glutathione-requiring Prostaglandin D Synthase, Prostaglandin-H2 D-isomerase, HPGDS, GSTS,

Prostaglandin D Synthase (PGDS) catalyzes the conversion of prostaglandin (PG) H2 to PGD2, which is a major prostanoid produced in a variety of tissues. Two types of PGDS have been isolated; the glutathione-dependent hematopoietic PGDS (HPGDS) and the glutathione-independent lipocalin-type PGDS (1). HPGDS is a cytosolic enzyme that is expressed in mast cells and antigen presenting cells (2, 3). It is the only mammalian member of the class Sigma glutathione S-transferase, showing a broad specificity towards standard transferase substrates (4). The PGD2 produced by HPGDS is involved in many physiological processes such as maintaining body temperature, promotion of sleep, inhibition of platelet aggregation and bronchoconstriction (5). It also functions in immune response and acts as a mediator in allergy and inflammation (6). HPGDS-specific inhibitors may be therapeutically useful anti-allergic and anti-inflammatory drugs.\n\nSource: \nRecombinant corresponding to aa1-199 from human Hematopoietic Prostaglandin D Synthase, fused with 6-His tag at C-terminal, expressed in E. coli.\n\nMolecular Weight: \n~64kD\n\nEndotoxin Level: \n(same/less than)1EU/1ug (LAL)\n\nBiological Activity:\nMeasured by its ability to hydrolyze the donor substrate GDP-fucose. The specific activity is >0.75pmoles/min/ug, as measured under the described conditions.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9053-25R

Size

10ug

Form

Supplied as a liquid in Tris, sodium chloride.

Purity

~95% (SDS-PAGE)

References

1. Urade, Y. and Eguchi, N. (2002) Prostaglandins Other Lipid Mediat. 68:375. 2. Urade, Y. et al. (1990) J. Biol. Chem. 265:371. 3. Urade, Y. et al. (1989) J. Immunol. 143:2982. 4. Jowsey, I. R. et al. (2001) Biochem. J. 359:507. 5. Kanaoka, Y. and Urade, Y. (2003) Prostaglandins Leukot. Essent. Fatty Acids 69:163. 6. Oguma, T. et al. (2008) Allergol. Int. 57:307.