PUMA (R169) pAb

PUMA (Bcl-2 binding component 3, JFY1, PUMA/JFY1) is a mitochondrial pro-apoptotic Bcl-2 homology domain (BH3)-only protein that induces rapid apoptosis through a Bax- and mitochondria-dependent pathway. The PUMA gene encodes four proteins originating from different splice variants of the same transcript: PUMA(alpha), (beta), (gamma) and (delta). Both PUMA(alpha) and PUMA(beta) contain a BH3 domain, while PUMA(gamma) and PUMA(delta) lack this domain. The BH3 domain is essential for binding of PUMA(alpha) and PUMA(beta) to Bcl-2 or Bcl-xL. PUMA is an initiator of (gamma)-radiation apoptosis and glucocorticoid-induced apoptosis in lymphoid cells in vivo. Bcl-2 family members generally regulate apoptosis and transmit death signals to mitochondria. Members of this family include both pro- and anti-apoptotic proteins that share homologous sequences known as Bcl-2 homology domains (BH1-4). The BH3 proteins, BID, NOXA, PUMA, NBK, Bim and Bad, are all pro-apoptotic and share sequence homology within the amphipathic (alpha)-helical BH3 region.

Prices direct from Bioworld Technology, Inc.

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

BS2922

Size

100ug/100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 132-183 of Human PUMA.

Species

PUMA (R169) pAb detects endogenous levels of PUMA protein.

Dilutions

WB: 1:500~1:1000

Swiss Prot

Q9BXH1

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 20 kDa

Alternative Names

BBC 3 antibody \nBBC3 antibody \nBCL 2 binding component 3 antibody \nBCL2 binding component 3 antibody \nJFY 1 antibody \nJFY1 antibody \np53 up regulated modulator of apoptosis antibody \np53 Upregulated Modulator of Apoptosis antibody \nPUMA alpha antibody \nPUMA/JFY1 antibody