Home  >  Products  >  Purification Media, BioSeparation(TM) Affinity, Protein A Beads (Agarose)

Purification Media, BioSeparation(TM) Affinity, Protein A Beads (Agarose)

Cat no: P9100-75


Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
The origin of Protein A is from the bacteria Staphylococcus aureus. Protein A is capable of binding most classes and subclasses of immunoglobulins from goat, rabbit, mouse and human. Protein A beads are produced using genetically engineered form of Protein A. Nonessential regions have been removed while leaving the immunoglobulins binding sites intact. The recombinant Protein A has been immobilized to agrose beads using TargetLock chemistry, in order to create a more stable product for the affinity purification and immunoprecipitation of various mammalian immunoglobulins. Protein A beads are being routinely used for the isolation of IgG from several species of mammals. The binding of Protein A to IgGs vary between animal species and between IgG subclasses within the same species. Major limitation of protein A lies with the weak binding it presents towards mouse IgG1, a common IgG subclass. Despite that, Protein A possesses useful properties that make it a popular choice for the isolation of most types of IgGs. Protein A binds IgGs through the Fc region of the molecules leaving the Fab region available for binding the antigen. Protein A Beads are commomnly used for antibodies purification and for the isolation of immune complexes by Immunoprecipitation. Protein A Beads Specifications: Matrix: Sepharose CL-4B Coupling method: TargetLock. Protein A Density: 2-4mg/ml of resin Binding Capacity: ~4-8mg rabbit IgG per ml of resin Mean bead size: 40 -165um Bead structure: Highly cross-linked spherical agarose, 4% Max back pressure: 0.3 MPa, 3 bar Max. flow rate: 4 ml/min/cm2 Recommended flow rate: 1-3 ml/min/cm2 Stability of the matrix: pH 2-11 Storage and Stability: May be stored at 4 degrees C. Do not freeze. Stable for at least 6 months.
Catalogue number: P9100-75
Conjugates: Agarose
Size: 1ml
Form: Supplied as a suspension in PBS, pH 7.4, 0.1% sodium azide.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*

Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave