Purification Media, BioSeparation(TM) Affinity, Protein A Beads (Agarose)

The origin of Protein A is from the bacteria Staphylococcus aureus. Protein A is capable of binding most classes and subclasses of immunoglobulins from goat, rabbit, mouse and human.\nProtein A beads are produced using genetically engineered form of Protein A. Nonessential regions have been removed while leaving the immunoglobulins binding sites intact. The recombinant Protein A has been immobilized to agrose beads using TargetLock chemistry, in order to create a more stable product for the affinity purification and immunoprecipitation of various mammalian immunoglobulins.\n\nProtein A beads are being routinely used for the isolation of IgG from several species of mammals. The binding of Protein A to IgGs vary between animal species and between IgG subclasses within the same species. Major limitation of protein A lies with the weak binding it\npresents towards mouse IgG1, a common IgG subclass. Despite that, Protein A possesses useful\nproperties that make it a popular choice for the isolation of most types of IgGs. Protein A binds IgGs through the Fc region of the molecules leaving the Fab region available for binding the antigen. Protein A Beads are commomnly used for antibodies purification and for the isolation of immune complexes by Immunoprecipitation.\n\nProtein A Beads Specifications:\n\nMatrix: \nSepharose CL-4B\n\nCoupling method: TargetLock.\n\nProtein A Density: \n2-4mg/ml of resin\n\nBinding Capacity: \n~4-8mg rabbit IgG per ml of resin\n\nMean bead size: \n40 -165um\n\nBead structure: \nHighly cross-linked spherical agarose, 4%\n\nMax back pressure: \n0.3 MPa, 3 bar\n\nMax. flow rate: \n4 ml/min/cm2\n\nRecommended flow rate: \n1-3 ml/min/cm2\n\nStability of the matrix: \npH 2-11\n\nStorage and Stability:\nMay be stored at 4 degrees C. Do not freeze. Stable for at least 6 months.

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