Goat Anti-Mouse IgG (whole molecule) was affinity purified on Mouse IgG affinity column using IgG isolated from pooled normal Mouse sera. Eluted antibodies were further purified by Protein G column in order to separate IgGs from other classes of antibodies. Purified Antibodies were coupled to Sepharose Beads.
Anti-Mouse Beads Specifications:
Matrix: Sepharose CL-4B
Coupling method: TargetLock
Coupling density: 0.7-1.2mg/ml of Goat anti Mouse IgG
Mouse IgG binding capacity: (same/more than)0.6mg/ml of beads
Mean bead size: 40 -165um
Range of pH values allowed: 3.0-11.0
Bead structure: Highly cross-linked spherical agarose, 4%
Max back pressure: 0.3MPa, 3 bar
Max. flow rates: 4ml/min/cm2
Recommended flow rate: 1-3ml/min/cm2
Storage: 4 degrees C in PBS pH 7.4, 0.3N NaCl added with 01% (w/v) azide as a preservative.
Storage:
Store at 4 degrees C in PBS, pH 7.4, 0.3N sodium chloride, 0.1% sodium azide..
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