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Pyruvate Dehydrogenase (C-term) mAb

Pyruvate Dehydrogenase (C-term) mAb

Cat no: MB0082

Supplier: Bioworld Technology, Inc.
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The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Catalogue number: MB0082
Reactivities: Human, Mouse
Hosts: Mouse
Applications: Western Blot
Size: 100ug/100ul
Swiss prot: P08559
Dilutions: WB: 1:1000 ICC: 1:100~200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic peptide, corresponding to the C-terminus of Human Pyruvate Dehydrogenase.
Species: This antibody detects endogenous levels of Pyruvate Dehydrogenase (C-terminus) and does not cross-react with related proteins
Molecular weight: Predicted band size:43KDa Observed band size:43KDa
Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: PDHA; PHE1A; PDHCE1A; PDHE1-A type I;
Additional info: For research use only, not for use in diagnostic procedure.

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