RANGAP1, NT (RANGAP1, KIAA1835, SD, Ran GTPase-activating protein 1) (APC)

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\nImmunohistochemistry: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

040827-APC

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Zhang, J., et al. Biochem. Biophys. Res. Commun. 375(2):252-255(2008)\nZuccolo, M., et al. EMBO J. 26(7):1853-1864(2007)\nEwing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :\nVertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006)\nVertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006)

Additional Info

Human

Alternative Names

RANGAP1, KIAA1835, SD, Ran GTPase-activating protein 1