RBBP7 (Nucleosome-remodeling Factor Subunit RBAP46, Histone Acetyltransferase Type B Subunit 2, Retinoblastoma-binding Protein p46, Retinoblastoma-binding Protein 7, RBBP-7, Histone-binding Protein RBBP7, RBAP46)

Retinoblastoma-associated proteins 46 and 48 (RBAP46 and RBAP48; also known as RBBP7 and RBBP4) were first characterized in human cells as proteins that bind to the retinoblastoma (Rb) tumor suppressor protein (1). Since then, these proteins have been shown to be components of many protein complexes involved in chromatin regulation, including the chromatin assembly factor 1 (CAF-1) complex and type B histone acetyltransferase complex HAT1, both of which function in chromatin assembly during DNA replication (2,3). RBAP46 and RBAP48 are also found in the nucleosome remodeling factor complex NURF, the nucleosome remodeling and histone de-acetylation complex NuRD, and the Sin3/HDAC histone de-acetylation complex (4-7). More recently, RBAB46 and RBAP48 were identified as components of the polycomb repressor complex PRC2, which also contains EED and Ezh2 (8). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target these chromatin remodeling, histone acetylation, and histone de-acetylation complexes to their histone substrates (3).\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence (IF-IC): 1:600\nWestern Blot: 1:1000 \nImmunoprecipitation: 1:50 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R1665-35D

Size

100ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP, Prc

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1.Qian, Y.W. et al. (1993) Nature 364, 648-52. 2.Verreault, A. et al. (1996) Cell 87, 95-104. 3.Verreault, A. et al. (1998) Curr Biol 8, 96-108. 4.Barak, O. et al. (2003) EMBO J 22, 6089-100. 5.Zhang, Y. et al. (1999) Genes Dev 13, 1924-35. 6.Zhang, Y. et al. (1997) Cell 89, 357-64. 7.Kuzmichev, A. et al. (2002) Genes Dev 16, 2893-905. 8.Cao, R. et al. (2002) Science 298, 1039-43.

Additional Info

Recognizes endogenous levels of total human RBAP46. Species Crossreactivity: Equine, monkey, mouse, porcine and rat. Does not cross-react with the RBAP48 protein.