RCC1, phosphorylated (Ser11) (Rgulator of chromosome condensation 1, Cell cycle regulatory protein, CHC1, Chromosome condensation protein 1, RCC1-I, Regulator of chromosome condensation)

The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle and nuclear transport (1,2). Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP- bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. RCC1 (regulator of chromatin condensation 1) is the only known RanGEF (3). RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally (4,5). Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation (5,6) and methylation (7). RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis (8). In mitosis RCC1 is phosphorylated at serine 11, possibly by cyclinB/cdc2 (9-11). This phosphorylation may play a role in RCC1 interaction with chromatin and its RanGEF activity (12). \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% \n Tween-20 at 4 degrees C with gentle shaking, overnight. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R1260-04

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44, 2. Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84, 3. Moore, W. et al. (2002) Curr Biol 12, 1442-7, 4. Ohtsubo, M. et al. (1989) J Cell Biol 109, 1389-97, 5. Li, H.Y. and Zheng, Y. (2004) Genes Dev 18, 512-27, 6. Hutchins, J.R. et al. (2004) Curr Biol 14, 1099-104, 7. Chen, T. et al. (2007) Nat Cell Biol 9, 596-603, 8. Wong, C.H. et al. (2009) Nat Cell Biol 11, 36-45, 9. Horiike, Y. et al. (2009) Mol Biol Rep 36, 717-23, 10. Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7, 11. Hood, F.E. and Clarke, P.R. (2007) J Cell Sci 120, 3436-45, 12. Hutchins, J.R. et al. (2004) Curr Biol 14, 1099-104.

Additional Info

Recognizes human Phospho-RCC1when phosphorylated at Ser11. Species crossreactivity: monkey.