RGS10 pAb

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. In mammals, G protein (alpha), (beta) and (gamma) polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their (alpha) subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four G(alpha) GTPase-activating proteins (GAPs) have been identified and are designated RGS1 (regulator of G protein signaling), RGS4, RGS10 and GAIP (G(alpha)-interacting protein). Each of these proteins has been shown to deactivate specific G(alpha) isoforms by increasing the rate at which they convert GTP to GDP. RGS1, RGS4 and GAIP bind tightly to and exhibit GAP activity towards G(alpha) i, G(alpha) o and G(alpha) t, but not G(alpha) s. RGS10 increases the GTP hydrolytic activity of several members of the G(alpha) i sub-family, including G(alpha) i-3, G(alpha) z and G(alpha) o.

Prices direct from Bioworld Technology, Inc.

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

BS5895

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 357-402 of Human RGS10.

Species

RGS10 pAb detects endogenous levels of RGS10 protein.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200

Swiss Prot

O43665

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul