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Rubella, E1 Mosaic, Recombinant (German Measles)

Cat no: R9700-23J

Rubella, E1 Mosaic, Recombinant (German Measles)

Rubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It is an enveloped (toga=cloak), non-segmented, positive sense, RNA virus and replicates in the cytoplasm. It consists of 3 structural proteins; E1,E2 membrane bound glycoproteins, and C capsid protein.\n\nE1 envelope glycoprotein is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and endocytosis, acidification of the endosome induces dissociation of E1/E2 heterodimer and trimerization of the E1 subunits. This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in the cytoplasm after cell and viral membrane fusion. The E1 cytoplasmic tail modulates virus release, and the tyrosines residues are critical for this function.\n\nSource: \nRecombinant corresponding to Rubella virus glycoprotein E1 immunodominant fragments, aa157-176, 213-239, 374-390 fused to 6-His-tag expressed in E. coli.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R9700-23J

Size

1mg

Applications

ELISA, WB

Form

Supplied as a liquid in PBS, 1mM HCl, 0.1% sodium azide.

Purity

~80% (SDS-PAGE)

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