Scrub typhus, Human, IgG BioAssay(TM) ELISA Kit (Orientia tsutsugamushi, OT, Rickettsia)

The Scrub Typhus Detect IgG ELISA test for exposure to Orientia tsutsugamushi (OT; formerly Rickettsia) is an ELISA assay system for the detection of IgG antibodies in human plasma/serum to OT derived recombinant antigen (1-10). This test is to aid in the diagnosis of human exposure to OT species. It is not intended to screen blood or blood components, and is for research use only. Not for use in diagnostic procedures.\n\nSummary:\nScrub Typhus is an infectious disease that is caused by Orientia tsutsugamushi (formerly Rickettsia), a tiny parasite about the size of bacteria that belongs to the family Rickettsiaceae. A bite from the larval trombiculid mite, a parasite of rodents, will transmit the disease. An ulcer of the skin is characteristic of a bite from a trombiculid mite, followed by symptoms including fever, a spotted rash on the torso, and swelling of the lymph glands. Scrub typhus generally occurs after exposure to areas with secondary (scrub) vegetation, which is where its name is derived from. However, the disease can also be prevalent in sandy, mountainous, and tropical areas. Scrub Typhus is a world wide illness, but particular to South East Asia and the Western Pacific. It accounts for approximately 20% of fever in some regions in South East Asia, where it is endemic. Illness lasts for a period of 10 to 12 days after the initial bite. With therapy, the fever will break within 36 hours, but if left untreated, complications or death may occur.\n\nPrinciple of the Test\nThe Scrub Typhus Detect ELISA system for IgG Test is a qualitative, membrane-based immunoassay for the detection of IgG antibodies to O. tsutsugamushi (OT) in serum. Wells of each plate have been coated with unique recombinant antigen mix. During testing, the serum sample is diluted in sample diluent and applied to each well. After incubation and washing, the wells are treated with IgG enzyme conjugate (HRP). After a second incubation and washing step, the wells are incubated with the tetramethylbenzidine (TMB) substrate. An acidic stopping solution is then added and the degree of enzymatic turnover of the substrate is determined by absorbance measurement at 450nm. The absorbance measured is directly proportional to the concentration of IgG antibodies to OT present. A set of positive and negative controls are provided as internal controls. These are provided to monitor the integrity of the kit components.\n\nFactor Tolerance \nNegative Control (NC) OD <0.200 \nPositive Control (PC) OD \n >0.500 \nDiscrimination Capacity (RPC/NC)(same/more than)5.0\n\nKit Components:\nS8099-01A: Microtiter Plate, 1x96 wells \nS8099-01B: Sample Dilution Buffer, 2x25ml \n*S8099-01C: Scrub Typhus IgG Positive Control, 1x30ul \n*S8099-01D: Scrub Typhus Negative Control, 1x50ul \nS8099-01E: Enzyme Conjugate (HRP), 1x12ml \nS8099-01F: Wash Buffer 10x, 1x120ml \nS8099-01G: HRP Wash Solution, 1x20ml \nS8099-01H: TMB Substrate:,1x12ml \nS8099-01J: Stop Solution, 1x6ml \n\nStorage and Stability:\nAliquot *S8099-01C and *S8099-01D and store at -20 degrees C. Store all other components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S8099-01

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. Kelly, D. J., et al; 2001. The present and past challenge of rickettsial disease to military medicine and public health. Clin Infect Dis 2002;34(suppl 4):s145-s169. 2. Weddle, J. R., et al; 1995. Effectiveness of a dot-blot immunoassay of anti-Rickettsia tsutsugamushi antibodies for serologic analysis of scrub typhus. Am. J. Trop. Med. Hyg. 53:43-46, 12. 3. Kelly, D. J., et al; 1990. Detection of Rickettsia tsutsugamushi by gene amplification using polymerase chain reaction techniques. Ann. N. Y. Acad. Sci. 590:564-571.\n4. Tamura, A.,et al; 1985 Analysis of polypeptide composition and antigenic components of Rickettsia tsutsugamushi by polyacrylamide gel electrophoresis and immunoblotting. Infect. Immum. 48:671-675\n5. Ohashi, N., A. Tamura, M. Ohta, and K. Hayashi. 1989. Purification and partial characterization of a type-specific antigen of Rickettsia tsutsugamushi. Infect. Immun. 57:1427-1431. 20.Eisemann, C. S., and J. V. Osterman. 1981. 6. Kim, I-S., et al; 1993. High-level expression of a 56- kilodalton protein gene (bor56) of Rickettsia tsutsugamushi Boryong and its application to enzyme- linked immunosorbent assays. J. Clin. Microbiol. 31:598-605. 7. Kim, I-S., et al;. 1993. Rapid diagnosis of scrub typhus by a passive hemagglutination assay using recombinant 56-kilodalton polypeptide. J. Clin. Microbiol. 31:2057-20606.\n8. Ching, W-M., et al; 1998.Expression and refolding of truncated recombinant major outer membrane protein antigen (r56) of Orientia 25.Land MV, et al; (2000). Evaluation of a Commercially-Available Recombinant Protein ELISA for Detection of Antibodies Produced in Scrub Typhus Rickettsial Infections. J. Clin. Micro. 38:2701-2705 9. Ching, WM. et al; 2000. Early Diagnosis of Scrub Typhus with a Rapid Flow Assay Using Recombinant Major Outer Membrane Protein Antigen (r56) of Orientia tsutsugamushi. Clin. Diag. Lab. Immunol. 8:409-414. 10. Ohashi, N., et al; 1992. Diversity of immunodominant 56-kD type- specific antigen (TSA) of Rickettsia tsutsugamushi. Sequence and comparative analyses of the genes encoding TSA homologues from four antigenic variants. J. Biol. Chem. 267:12728-12735