Semaphorin 4A (Semaphorin-4A, Semaphorin-B, Sema B, SEMA4A, SEMAB, SEMB, UNQ783/PRO1317) (APC)

Semaphorin 4A (Sema4A; previously known as semB) is a class 4 transmembrane semaphorin with activity in the immune and nervous systems. The extracellular domain of human Sema4A shares 87%, 87%, 86%, and 85% aa sequence identity with mouse, rat, bovine, and canine Sema4A, respectively, and shares 32-37% aa identity with other human Sema4 family members. Sema4A was first described as a molecule that enhances T cell activation and interacts with TIM-2 (T cell immunoglobulin and mucin domain-2. Mice with targeted disruption of Sema4A show defects in dendritic cell-mediated T cell priming and Th1 responses. Roles for Sema4A have also been identified inthe brain, the endothelium, and the eye. It mediates hippocampal neuron growth cone collapse in vitro through interaction of the sema domain with Plexin B1. Interaction of Sema4A with endothelial cell Plexin D1causes opposition to the angiogenic, proliferative, chemotactic, and integrin-mediated adhesive actions of VEGF. The retina of Sema4A-/- mice shows severe degeneration, and mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nAbsorption: 620-650nm\n\nEmission: 660-670nm\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

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SPECIFICATIONS

Catalog Number

S0668-54C

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1

References

1. Kumanogoh, A. et al. (2003) J. Cell Sci. 116:3463. 2. Kumanogoh, A. et al. (2002) Nature 419:629. 3. Kumanogoh, A. et al. (2005) Immunity 22:305. 4. Yukawa, K. et al. (2005) Int. J. Mol. Med. 16:115. 5. Toyofuku, T. et al. (2007) EMBO J. 26:1373. 6. Rice, D.S. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:2767. 7. Abid, A. et al. (2007) J. Med. Genet. 43:378.

Additional Info

Recognizes human Semaphorin 4A.