Semenogelin I (Semenogelin-1, SEMG1, SEMG, SGI, CT103, dJ172H20.2, FLJ78262, MGC14719, Semen Coagulating Protein, Semenogelin, SEMG)

Semenogelin I (SgI) and Semenogelin II (SgII) are the major seminal vesicle secreted proteins in human semen (reviewed in Lundwall, 2002 and Bonilha et al, 2006). SgI and SgII, in semen both originate from the glandular epithelium of the seminal vesicles which secrete them at high concentrations. SgII is also secreted by the epididymis at lower concentrations. SgI and SgII interact both non-covalently and covalently by disulphide bridges to instantly form a gel-like coagulum upon ejaculation. Fibronectin is also a key component of the coagulum. The gel structure dissolves spontaneously within minutes after ejaculation as a result of proteolytic degradation of SgI:SgII by prostatic serine protease (PSA) and other proteases which cleave SgI:SgII into fragments. The high concentration of SgI:SgII in human semen led to the concept of SgI:SgII as a potentially useful marker for semen identification (reviewed in Pang and Cheung, 2006). Whereas SgI:SgII were originally identified as the major components present in seminal vesicle secretion, they are now known to be expressed in a number of tissues. The list includes seminal vesicles, epididymis, vas deferens, prostate, skeletal muscle, kidney, colon, trachea, lung, breast and retina as well as in several malignant tissues and cell lines (reviewed in Lundwall, 2002 and Bonilha et al, 2006). The non-genital expression of SgI:SgII suggests that these proteins also have functions that are unrelated to sperm. Human Sg1 is a non-glycosylated protein of 439aa with a molecular weight of ~50kD. A few percent of the worlds population also carry an allele that gives rise to a truncated SgI molecule with a molecular mass of 43kD. Human SgII is a 559aa protein with a molecular weight of ~63kD. SgII has a potential site for N-linked glycosylation and approximately half of the molecules in human semen are glycosylated yielding two SgII species with a different of ~5kD. SgI has a single Cys residue and SgII has two Cys residues, and the molecules can exist as covalent homo- and heteromultimers.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:2000\nImmunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000\nImmunoprecipitation: 1:50-1:200\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMale sex organs: seminal vesicles\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S0670-50A

Size

50ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Lundwall A, A Bjartell, AY Olsson, and J Malm. 2002. Semenogelin I and II, the predominant human seminal plasma proteins, are also expressed in non-genital tissues. Molecular Human Reproduction 8:805-810. 2. Bonilha VL, ME Rayborn, K Shadrach, A Lundwall, J Malm, SK Bhattacharya, JW Crabb and JG Holly Field. 2006. Characterization of semenogelin proteins in the human retina. Experimental Eye Research 83:120-127. 3. Pang BCM and BKK Cheung. 2006. Identification of human semenogelin in membrane strip test as an alternative method for the detection of semen. Forensic Science International doi:10.1016/j.forsciint.2006.07.021

Additional Info

Recognizes rat Semenogelin II. Species Crossreactivity: gerbil, mouse, human.

Alternative Names

Alpha-inhibin-31, Alpha-inhibin-92, Seminal Basic Protein