Serotonin Receptor 3A (5-HT3A, 5-HT3-A, HTR3A, 5-Hydroxytryptamine (Serotonin) Receptor 3A, 5-Hydroxytryptamine Receptor 3, 5HT3, 5-HT-3, 5-HT3R, HTR3, Serotonin-gated Ion Channel Receptor)

Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems (1). The 5-HT3 receptors are distinguished from other 5-HT receptors by their structure and function. 5-HT3 receptors span the membrane only four times and are ligand-gated, cation-selective ion channels that mediate neuronal excitation and exocytotic release of neurotransmitters within the peripheral and central nervous systems (2,3). Functional studies done on isolated tissues have revealed heterogeneity of this receptor. The human 5-HT3R-As cDNA contains an open reading frame of 1434 base pairs predicting a 23-residue signal peptide and a mature receptor subunit of 455aa (4). The human subunit exhibits 85% and 84% aa sequence identity to the mouse (5) and rat (6) receptors, respectively. Anti-5-HT3R polyclonal antibody shows immunoreactivity in the forebrain (olfactory regions, hippocampal formation and amygdala), sensory and motor nuclei of brainstem and spinal cord (7).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nBrain\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1001-19K

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity Chromatography.

Isotype

IgG

References

1. Petrouka, SJ. Trends Neurosci. 11: 486-500 (1998). 2. Derkach, V., Surprenant, A., North, RA. Nature 339: 706-709 (1989). 3. Maricq, AV., Peterson, AS., Brake, AJ., Myers, RM., Julius, D. Science 254: 432-437 (1991). 4. Belleli, D., Balcarek, JM., Hope, AG., Peters, JA., Lambert, JJ., Blackburn, TP. Mol. Phrmacol. 48: 1054-1062 (1992). 5. Hope, AG., Downie, DL., Sutherland, L., Lambert, JJ., Peters, JA, Burchell, B. Eur. J. Pharmacol. 245: 87-192 (1993). 6. Johnson, DS. And Heinemann, SF. Soc. Neurosci. (abstract) 18: 249 (1992). 7. Morales, M., Battenberg, E., de Lecca, L., Sanna, PP., Bloom, F. Mol. Brain Res. 36: 251-260 (1996).

Additional Info

Recognizes rat 5-HT3AR. Species Crossreactivity: human and mouse.

Alternative Names

5HT3 Serotonin Receptor