Sex Hormone-Binding Globulin (SHBG, Sex Steroid-binding Protein, SBP, Testis-specific Androgen-binding Protein, BP, Testosterone-estradiol-binding Globulin, TeBG, Testosterone-estrogen-binding Globulin)

SHBG is a protein of mol wt 90kD (402aa) containing two subunits. SHBG transports the sex steroids testosterone, DHT and estradiol in the blood. Serum levels are increased during pregnancy, in women taking estrogens, and in men over the age of 50. Levels are reduced after menopause and in response to treatment with androgens or corticosteroids. Diagnostic uses include polycystic ovary disease and tests for liver and thyroid function Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:10,000-1:100,000 \nWestern Blot: 1:1000-1:5000 using chemiluminescence\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1012-52F

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophillized powder from PBS, 0.1% BSA. Reconstitute in 100ul sterile PBS.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1

References

1. Hammond GI (1987) FEBS Lett. 215, 100- 104. 2. Hammond GI (1986) J. Steroid Biochem. 24, 815-824. 3. Powers SG (1992) J. Clin. Endocrinol. Metab. 75 (4), 1066-1070. 4. Grishkovskaya,I (2002) J. Biol. Chem. 277 (35), 32086-32093.

Additional Info

Recognizes human Sex Hormone-Binding Globulin.