SF3B1, NT (SF3B1, SAP155, Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155kD subunit, Spliceosome-associated protein 155) (AP)

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\nImmunohistochemistry: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

041612-AP

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Alkaline Phosphatase.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Corsini, L., et al. J. Biol. Chem. 284(1):630-639(2009)\nTanuma, N., et al. J. Biol. Chem. 283(51):35805-35814(2008)\nPessa, H.K., et al. Proc. Natl. Acad. Sci. U.S.A. 105(25):8655-8660(2008)\nKuwasako, K., et al. Proteins 71(4):1617-1636(2008)\nAvila, M.L., et al. Biochem. Biophys. Res. Commun. 364(1):26-32(2007)

Additional Info

Human

Alternative Names

SF3B1, SAP155, Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155kD subunit, Spliceosome-associated protein 155