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SF3B1 pAb

Cat no: BS5919

SF3B1 pAb

SF3b is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3b contains the spliceosomal proteins SAP 49, SAP 130, SAP 145, and SAP 155. SAP 130, SAP 145, and SAP 155 are present in a protein complex in HeLa nuclear extracts and associate with one another. While SAP 155 and SAP 130 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.

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SPECIFICATIONS

Catalog Number

BS5919

Size

100ug/100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 134-179 of Human SF3B1.

Species

SF3B1 pAb detects endogenous levels of SF3B1 protein.

Dilutions

WB: 1:500~1:1000

Swiss Prot

O75533

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 155 kDa

Alternative Names

Splicing factor 3B subunit 1; Pre-mRNA-splicing factor SF3b 155 kDa subunit; SF3b155; Spliceosome-associated protein 155; SAP 155; SAP155

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