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SF3b155/SAP155 (Pre-mRNA-splicing factor SF3b 155kD subunit, Prp10, PRP10, PRPF10, SAP155, SAP 155, SF3b155, Spliceosome-associated protein 155, Splicing factor 3B subunit 1)

Cat no: S0096-23B

SF3b155/SAP155 (Pre-mRNA-splicing factor SF3b 155kD subunit, Prp10, PRP10, PRPF10, SAP155, SAP 155, SF3b155, Spliceosome-associated protein 155, Splicing factor 3B subunit 1)

SAP155 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of the SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. It may also be involved in the assembly of the 'E' complex. SAP155 also belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. \n\nApplications:\nSuitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nImmunoprecipitation: 2-5ug/mg\nWestern Blot: 1:2000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0096-23B

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.

P Type

Pab

Purity

Purified by Immunoaffinity chromatography.

Additional Info

Recognizes human SF3b155.

Read more on Supplier website

SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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