SF3B2, ID (SF3B2, SAP145, Splicing factor 3B subunit 2, Pre-mRNA-splicing factor SF3b 145kD subunit, SF3b150, Spliceosome-associated protein 145) (Biotin)

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. [provided by RefSeq].\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

041614-Biotin

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Matsuoka, S., et al. Science 316(5828):1160-1166(2007)\nEwing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :\nOlsen, J.V., et al. Cell 127(3):635-648(2006)\nOlsen, J.V., et al. Cell 127(3):635-648(2006)\nTerada, Y., et al. Mol. Cell. Biol. 26(21):8149-8158(2006)

Additional Info

Human

Alternative Names

SF3B2, SAP145, Splicing factor 3B subunit 2, Pre-mRNA-splicing factor SF3b 145kD subunit, SF3b150, Spliceosome-associated protein 145