SFRP3 (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1)

Secreted Frizzled Related Protein 3 (SFRP3) was originally identified in bovine cartilage for its chondrogenic ability. Human, mouse, chick and Xenopus clones have also been isolated. SFRP3 is often referred to as FRZB, other names include Fritz, Frzb1, and FRP-3. At the amino acid sequence level, SFRP3 is highly conserved. The human protein shares 77% identity with Xenopus, 92% with mouse, and 94% with bovine proteins. Human SFRP3 is strongly expressed in the developing appendicular skeleton, and cartilage of craniofacial bones. As determined by Northern blot of adult tissues, it is strongly detected in heart and placenta, as well as the brain, skeletal muscle, kidney and pancreas.\nThe N-terminal portion of the human protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals. The similarity of SFRP3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them. SFRP3 was subsequently shown to be a soluble antagonist of Wnt signals. It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts. Ectopic expression of SFRP3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA.\n\nApplications: \nSuitable for use in Direct ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1ug/ml with the appropriate secondary reagents to detect recombinant human and mouse SFRP3.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1012-88

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography. Endotoxin: <0.1EU/ug (LAL)

Isotype

IgG

References

1. Hoang, et al., J. Biol. Chem. 271: 26,131 (1996). 2. Leyns, et al., Cell 88: 747 (1997). 3. Wang, et al., Cell 88: 757 (1997). 4. Mayr, et al., Mech. Dev. 63: 109 (1997). 5. Rattner, et al., PNAS 94: 2859 (1997).

Additional Info

Recognizes human SFRP3. Species Crossreactivity: mouse