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SG2NA (Striatin-3, calmodulin binding protein 3, Cell-cycle autoantigen SG2NA, GS2NA, S/G2 Antigen)

Cat no: S1009

SG2NA (Striatin-3, calmodulin binding protein 3, Cell-cycle autoantigen SG2NA, GS2NA, S/G2 Antigen)

SG2NA, also known as Striatin 3, was identified as a human cancer auto-antigen and has not been implicated in vesicular trafficking and signal tranduction. It is highly related to striatin and zinedin (striatin 4). It is a cytoplasmic scaffolding protein that binds to Calmodulin and PP2A A and C subunits as well as the human homolog of the yeast protein, Mob1, which appears to modulate PP2A activity.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000 detected SG2NA on 10ug of L6 lysates. Recgonizes SG2NA at ~93kD in Jurkat cell lysate. In some cell types, a band of ~70kD is also recognized, which may be an alternatively splliced form of SG2NA or another unknown protein.\nImmunoprecipitation: (same/more than)3ul\nImmunocytochemistry: 1:500\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nL6 cell lysate\n\nRecommended Secondary Antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo\nI1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1009

Size

100ul

Applications

ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.02% sodium azide, 40% glycerol.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Moreno, C.S., et al. (2001). J. Biol. Chem. 276:24253-24260.

Additional Info

Recognizes SG2NA at 93kD. Does not crossreact with striatin. Species Crossreactivity: human, rat, mouse.

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